论文部分内容阅读
应用ＧＰＰＣＲ 技术对４０ 例宫颈癌活检组织进行ＨＰＶ 感染的检测，发现有３５ 例为阳性（ 阳性率为８７％ ），同时利用ＰＣＲＳＳＣＰ（单纯ＤＮＡ构象多态性）分析技术，对３５ 例ＨＰＶ 阳性的宫颈癌组织进行Ｐ５３ 基因５ ～６外显子的检测，其突变率仅为２－８５ ％（１／３５），而在５ 例ＨＰＶ 阴性的宫颈癌组织中未检测到Ｐ５３ 基因突变，结果表明：Ｐ５３ 基因的突变在宫颈癌的发生过程中不起主要作用，并且与ＨＰＶ的感染无相关性。
Application of GP PCR technology in 40 cases of cervical cancer biopsy HPV infection was detected in 35 cases were positive (positive rate was 87%), using PCR-SSCP (simple DNA conformation polymorphism) analysis of 35 Cases of HPV-positive cervical cancer P53 gene 5-6 exon detection, the mutation rate of only 2-85% (1/35), and in 5 cases of HPV-negative cervical cancer tissue was not detected P53 gene The results showed that the mutation of P53 gene could not play a major role in the development of cervical cancer and had no correlation with HPV infection.