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The effect of hot isostatic pressing (HIP) treatment on the fatigue notch sensitivity of Ti-6Al-4V alloy fabricated by electron beam melting (EBM) was investigated.The results indicate that the fatigue notch sensitivity of the as-fabricated samples is much lower than that of HIPed samples.The variation of α lamella thickness and the distribution of pore defects have an effect on the fatigue strength in smooth and notched EBM Ti-6Al-4V samples,resulting in the divergence of the as-fabricated and HIPed samples on fatigue notch sensitivity.