随疟原虫研究工作的深入,为提取疟原虫的抗原或核酸,对恶性疟原虫的需要量大增。目前国内常用的三角烧瓶蜡烛缸法,很难满足上述工作的需要。因此需要建立简便实用的大量体外培养恶性疟原虫的方法。国外近年来使用复杂的自动换液等机械装置,国内目前尚无条件做到。为此我们建立了 With the further research work on malaria parasites, the demand for Plasmodium falciparum is greatly increased in order to extract antigens or nucleic acids of malaria parasite. At present, the commonly used triangular flask candle jar method, it is difficult to meet the needs of the above work. Therefore, there is a need to establish a simple and practical method for culturing P. falciparum in vitro. In recent years, foreign countries have used complicated automatic liquid changer and other mechanical devices, so China currently has no conditions to do so. For this we have established