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目的:探讨男性工人血浆mi RNA在非接噪组、接噪组和高频听损组之间的差异表达。方法 :30例男性工人分为非接噪组、接噪组和高频听损组,对其mi RNA的差异表达进行芯片初筛,再用q RT-PCR验证6个mi RNA是否存在差异表达,然后借助GO富集分析和KEGG信号通路分析预测mi RNA推定靶基因可能参与调控的生物学过程和功能。结果:与非接噪组比,接噪组血浆let-7d(P=0.002)、mi R-16(P<0.001)、mi R-24(P<0.001)、mi R-185(P<0.001)和mi R-451a(P=0.011)表达均显著下调;与接噪组相比,高频听损组血浆mi R-16(P<0.001)表达显著上调,且与芯片结果一致。结论:let-7d、mi R-16、mi R-24、mi R-185和mi R-451a可能参与调控机体对噪声刺激的反应过程,并且mi R-16有前景成为噪声导致高频听力损伤的生物标志。
Objective: To investigate the differential expression of plasma mi RNA in male workers between non-noise-canceling group, receiving noise group and high-frequency hearing loss group. Methods: Thirty male workers were divided into non-noise group, noise group and high-frequency hearing loss group, and their differential expression of mi RNA was preliminarily screened by chip. Then q-RT-PCR was used to verify whether six mi RNAs were differentially expressed , And then using GO enrichment analysis and KEGG signaling pathway analysis to predict the miRNA inferred target genes may participate in the regulation of biological processes and functions. RESULTS: Compared with the non-receiving group, the let-7d (P = 0.002), mi R-16 (P <0.001), mi R-24 ) And mi R-451a (P = 0.011), respectively. The expression of mi R-16 (P <0.001) in high-frequency hearing loss group was significantly up-regulated compared with the noise receiving group. CONCLUSIONS: let-7d, mi R-16, mi R-24, mi R-185 and mi R-451a may be involved in the regulation of the body’s response to noise stimuli, and mi R-16 is promising as a noise-induced hearing loss Biomarkers