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【目的】筛选能够利用甲醇的微生物菌株,并将其应用于甲醇测定的初步研究中。【方法】采用富集培养及固体平板分离法分离菌株;通过生理生化特征及16S rDNA序列分析进行菌株鉴定;以固定化细胞和溶氧仪组合成测定系统,测定了不同含量甲醇的响应时间以及溶氧变化与甲醇含量的关系。【结果】从山西清徐农用沼气池中分离得到一株能以甲醇为唯一碳源和能源生长的菌株M211,通过生理生化特征及16SrDNA序列分析,该菌株被鉴定为嗜有机甲基杆菌(Methylobacterium organophilium)。传感系统在0.02%-1%(V/V)甲醇含量范围内测定,响应时间小于20 min,检出限为0.27 mg/L,溶氧消耗量同甲醇含量呈线性关系,拟合系数(R2)为0.9986。该测定体系不易受其它干扰物质影响,检测结果与实际样品浓度一致。【结论】该测定体系具有较强的选择性,及良好的重现性和稳定性,具有较好的应用前景。
【Objective】 The objective of this study was to screen microbial strains that could utilize methanol and apply it in the preliminary study of methanol determination. 【Method】 The strains were isolated by enrichment culture and solid plate separation method. The strains were identified by physiological and biochemical characteristics and 16S rDNA sequence analysis. The immobilized cells and the dissolved oxygen analyzer were combined into a measuring system to determine the response time of methanol with different contents. Relationship between Dissolved Oxygen and Methanol Content. 【Result】 A strain M211 which could grow with methanol as sole carbon source and energy was isolated from Qingxu agricultural biogas digester in Shanxi. The strain was identified as Methylobacterium (Methylobacterium) by physiological and biochemical characteristics and 16S rDNA sequence analysis. organophilium. The sensory system was measured in the range of 0.02% -1% (V / V) methanol. The response time was less than 20 min. The limit of detection was 0.27 mg / L. The oxygen consumption was linear with the methanol content. R2) is 0.9986. The measurement system is not susceptible to other interfering substances, the test results and the actual sample concentration. 【Conclusion】 The assay system has strong selectivity, good reproducibility and stability, and has good application prospects.