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以大田栽培的三套杂交水稻组合汕优63,青优早和秀优57为材料,研究了低温(5℃)与光照(210 μmol m~(-2)s~(-1)PFD)对杂交水稻F_1及其亲本剑叶77K荧光的影响。结果表明:低温引起77K荧光参数Fo,Fm,Fv下降,可变荧光Fv的变化能反映三套杂交水稻组合间的抗冷性的差异。F_1的抗冷性居父母本之间。同时发现,在低光照下低温处理引起Fo,Fm,Fv上升,但未改变Fv/Fm,Fv/Fo的比值。证明在低温下,低于水稻生长光强210 μmol m~(-2)s~(-1)PFD的光照对PSⅡ的最初光能转化效率和光能传递速率影响不大,即对光合作用的光化学过程未产生光抑制现象。由此可见,取样于田间强光照下的水稻剑叶,在5℃和210 μmol m~(-2)s~(-1)PFD处理下,210 μmol m~(-2)s~(-1)PFD可能有利于水稻对低温的适应,从而减轻叶片的光化学过程的伤害程度。
The effects of low temperature (5 ℃) and light (210 μmol m -2 s -1 PFD) on the three hybrid rice combinations Shanyou 63, Qing Youzao and Xiuyou 57 were studied. Effect of 77K Fluorescence on Flag Leaves of Hybrid Rice F_1 and Its Parents. The results showed that under low temperature, the fluorescence parameters Fo, Fm and Fv of 77K decreased, and the variation of variable fluorescence Fv could reflect the difference of chilling tolerance between three hybrid rice combinations. F_1’s cold resistance among parents of this. At the same time, it was found that the low temperature treatment caused the increase of Fo, Fm and Fv in low light, but did not change the ratio of Fv / Fm and Fv / Fo. The results showed that the light energy of 210 μmol m -2 s -1 PFD lower than that of rice at low temperature had no significant effect on the initial light energy conversion rate and light energy transfer rate of PS Ⅱ, that is, the photochemistry of photosynthesis The process did not produce photoinhibition. The results showed that under the field illumination, the flag leaf of rice was sampled and treated with 210 μmol m -2 s -1 of PFD at 5 ℃ and 210 μmol m -2 s -1 PFD, PFD may be beneficial to the adaptation of rice to low temperatures, thereby reducing the extent of photochemical damage to the leaves.