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以抗药性突变菌株为筛选标记,采用稀释倒平板法,研究了内生细菌EBS05在小麦体内的定殖动态,并通过盆栽和大田试验研究了EBS05对小麦纹枯病的防治效果。结果表明,EBS05在小麦根内具有较强的定殖能力,且能从根部向茎、叶部转移,其定殖量与菌株的接种浓度呈正相关,当接种浓度为108 CFU/mL时,EBS05能在根、茎内有效定殖,并持续向叶内转移。以菌体浓度为108CFU/mL的发酵液进行浸种或灌根处理后,EBS05在根内定殖量始终大于同时期茎、叶内的定殖量;菌株在小麦根、茎和叶内的定殖数量均表现为接种初期逐渐增加,至接种后第10～12天达到最大值,随后逐渐降低。内生细菌EBS05对小麦纹枯病的防治效果优于对照药剂25 g/L咯菌腈(flu-dioxonil)悬浮种衣剂,带菌发酵液和除菌发酵液的盆栽防治效果分别达91.2%和88.2%,大田防治效果分别为66.3%和56.2%。
The resistant mutant strain was selected as the marker and the colonization dynamics of endophytic bacteria EBS05 in wheat was studied by dilution plate method. The control effect of EBS05 on wheat sheath blight was studied by pot and field experiments. The results showed that EBS05 had strong colonization ability in wheat roots and could be transferred from the roots to the stems and leaves, and its colonization amount was positively correlated with the inoculation concentration of the strains. When the inoculation concentration was 108 CFU / mL, EBS05 Can be effective in roots and stem colonization, and continue to transfer to the leaves. After soaking or rooting with the broth of 108CFU / mL, the colonization amount of EBS05 in the root was always higher than that of the stem and leaves in the same period. The colonization of the strain in wheat roots, stems and leaves The numbers showed a gradual increase from the beginning of inoculation to the maximum value on the 10th to 12th day after inoculation, and then gradually decreased. The control efficacy of endophytic bacteria EBS05 on wheat sheath blight was better than that of the control agent 25 g / L flu-dioxonil suspension coating, and the control effect of the bacterium broth and the sterilization broth was 91.2% and 88.2%. The control effect in the field was 66.3% and 56.2% respectively.