自阿片受体在动物和人的脑组织中的存在被证实以后,分离纯化阿片受体的研究十分引人注意。由于阿片受体含量极微,又难以从膜上溶脱,因而进展受阻。目前虽然获得用适当的去垢剂助溶的水溶性阿片受体,然产率较低,容易失活。本文报道用我们合成的氚标记的强效镇痛剂[~3H]羟甲芬太尼,即N-[1-(β-羟基-β-苯乙基)-3-甲基-4-哌啶基]-N-丙酰苯胺(简 Since the existence of opioid receptors in animal and human brain tissues has been demonstrated, the isolation and purification of opioid receptors is of great interest. As the opioid receptor content is extremely small and difficult to elute from the membrane, the progress is hindered. At present, although water-soluble opioid receptors that are solubilized with suitable detergents are obtained, yields are low and inactivation is readily possible. This paper reports the use of tritium-labeled potent analgesic [~ 3H] oxymetapane synthesized in our laboratory, ie N- [1- (β-hydroxy-β-phenylethyl) Pyridinyl] propionanilide (abbreviation