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作者介绍了一种简便、迅速又敏感的检测支原体新方法,它可通过污染的细胞培养上清液抑制~3H-TdR掺入到IL-2依赖的细胞毒性T细胞(CTLL)的量来定量测定污染的支原体,并能检测细胞是否已清除污染的支原体。抑制CTLL结合~3H-TdR的测定:取100μl细胞培养上清液加入96孔平底培养板
The authors describe a simple, rapid and sensitive new method for the detection of mycoplasma that can be quantitated by the amount of 3H-TdR incorporation into IL-2-dependent cytotoxic T cells (CTLL) in contaminated cell culture supernatants The contaminating mycoplasma is assayed and the cells are assayed for the presence of cleared Mycoplasma. Inhibition of CTLL binding ~ 3H-TdR determination: Take 100μl cell culture supernatant was added to 96-well flat-bottomed culture plates