论文部分内容阅读
[目的]研究浆液性卵巢肿瘤组织中芳香化酶P450(P450Arom)、雌激素受体α(ERα)的表达,以探索雌激素相关因子在浆液性卵巢癌中的作用。[方法]收集初次手术治疗确诊为浆液性卵巢癌患者49例,并以同期良性浆液性卵巢肿瘤患者50例作为对照。采用免疫组化法分别测定P450Arom、ERα蛋白在良性和恶性浆液性卵巢肿瘤组织中的表达,并以组织化学评分(H-score)法进行半定量分析。[结果]在良性和恶性浆液性卵巢肿瘤的上皮和间质中均无P450Arom表达。浆液性卵巢癌细胞ERα表达平均H-score分为1.52±0.47,显著性高于良性浆液性卵巢肿瘤的1.13±0.18,差异有统计学意义(t=5.24,P=0.00)。[结论]浆液性卵巢癌存在局部雌激素相关调节因子表达的改变,表现为P450Arom蛋白表达缺失、ERα蛋白表达上调。
[Objective] To investigate the expression of aromatase P450 (P450Arom) and estrogen receptor α (ERα) in serous ovarian tumors to explore the role of estrogen-related factors in serous ovarian cancer. [Method] Forty-nine patients with serous ovarian cancer who were diagnosed as primary surgical treatment were collected and compared with 50 patients with benign serous ovarian tumors in the same period. The expressions of P450Arom and ERαprotein in benign and malignant serous ovarian tumors were detected by immunohistochemistry and semi-quantitatively by histochemical method (H-score). [Results] There was no P450Arom expression in the epithelium and stroma of benign and malignant serous ovarian tumors. The average H-score of ERα expression in serous ovarian cancer cells was 1.52 ± 0.47, which was significantly higher than that of benign serous ovarian tumors (1.13 ± 0.18), the difference was statistically significant (t = 5.24, P = 0.00). [Conclusion] There is a change in the expression of local estrogen-related regulators in serous ovarian cancer. The expression of P450Arom protein is absent and ERα protein is up-regulated.