论文部分内容阅读
目的 探讨放烧复合伤小鼠腹腔巨噬细胞数量、形态及其IL 12基因表达变化的复合效应。方法 于单纯放射损伤 (5Gy)或放烧复合伤 (5Gy合并 12 %II度烧伤 )后不同时相点分离、计数小鼠腹腔巨噬细胞 ,观察其形态学变化 ,RT PCR方法检测其IL 12、p35和p40的基因表达水平。结果 ①同放射损伤组相比 ,放烧复合伤组早期巨噬细胞数量减少速度更快 ,减少程度更大 ,但后期恢复速度明显加快 ;②同放射损伤组相比 ,放烧复合伤组伤后早期溶解坏死的巨噬细胞较多 ,但在形态上更接近正常 ;③两伤组p35和p40伤后表达的变化趋势一致 ,但放烧复合伤组伤后各时相点两基因的表达量明显高于放射损伤组。结论 放烧复合伤在免疫细胞数量、形态和功能上具有“早期加重 ,后期减轻”的复合效应特点 ,提示“早期救治”在放烧复合伤救治中的重要性。
Objective To investigate the combined effect of peritoneal macrophages number, morphology and IL 12 gene expression in the burn-burn combined mice. Methods The peritoneal macrophages of mice were isolated at different time points after radiation injury (5Gy) or burn-burn combined injury (5Gy combined with 12% II degree burn) at different time points. Morphological changes were observed. The levels of IL-12 , P35 and p40 gene expression levels. Results ① Compared with radiation injury group, the number of early macrophages in burn-burn group decreased more rapidly and with a greater degree of reduction, but the recovery rate in the later period was significantly accelerated. ② Compared with radiation injury group, There were more macrophages in early stage after necrosis, but they were closer to normal in morphology. ③ The expression changes of p35 and p40 were similar in both groups, however, Significantly higher than the radiation injury group. Conclusion The combination of burn and burn injury has the characteristics of compound effect of “early treatment” and “late elimination” on the number, shape and function of immune cells, which indicates the importance of “early treatment” in the treatment of radiotherapy combined with burn.