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应用霍乱毒素Ｂ亚单位结合胶体金（ＣＢ－Ａｕ）逆行标认复合降钙素基因相关肽样免疫细胞化学反应（ＣＧＲＰ－ＬＩ）的方法，对发育过程中大鼠比目鱼肌（慢肌）和趾长伸肌（快肌）运动神经元群的ＣＧＲＰ－ＬＩ含量强度配布式样进行了分析。结果表明，大鼠快、慢肌运动神经元群之间的ＣＧＲＰ－ＬＩ含量强度在发育过程中即有着明显的不同，慢肌运动神经元群中含有较多ＣＧＲＰ－ＬＩ阴性和弱阳性运动神经元，而快肌运动神经元群含较多ＣＧＲＰ－ＬＩ强阳性的运动神经元，表明调质ＣＧＲＰ与运动神经元群所支配的肌肉功能活动方式密切相关。
The cholera toxin B subunit combined with colloidal gold (CB-Au) retrograde identification of compound calcitonin gene-related peptide immunocytochemistry (CGRP-LI) method, the development of rat soleus muscle (slow muscle) and CGRP-LI content intensity distribution pattern of long-toot extensor (fast muscle) motor neuron population was analyzed. The results showed that the intensity of CGRP-LI content between the groups of fast and slow motor neurons in rats was significantly different during development, and the group of slow muscle motor neurons contained more CGRP-LI negative and weak positive motor nerves Yuan, and fast muscle motor neurons contain more CGRP-LI strongly positive motor neurons, indicating that the modulation of CGRP and motor neuron population is dominated by muscle function activity is closely related.