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目的本实验旨在初步探讨A/Shanghai/4664T(H7N9)和A/Puerto Rico/8/34(H1N1)病毒感染BALB/c小鼠的发病特点,为H7N9致病机制与防治的研究提供数据支持。方法将同等剂量(5×103TCID50)流感病毒滴鼻感染BALB/c小鼠,分析其在感染后体重、肺指数、病毒载量和肺组织病理的变化情况。结果 H1N1PR8感染组和H7N9感染组小鼠在7 d内体重均持续减低,H1N1 PR8组较H7N9组降低更为明显;在感染后3 d H7N9组与PBS组肺指数无显著差异(P>0.05),但感染后7d H7N9组与PBS组相比显著增高(P<0.05);H1N1PR8组比H7N9组感染后3 d肺指数显著增高(P<0.05),但感染后第7天无显著差异(P>0.05)。H1N1 PR8组和H7N9组病毒载量在感染后第3天均显著升高,但两组无显著差异;第7天两组病毒载量均有所降低,但H7N9组病毒载量显著高于H1N1 PR8组(P<0.05)。H1N1 PR8在感染后3 d主要病理变化为炎症细胞浸润和水肿,而H7N9组主要轻度炎症细胞浸润;在感染后7 dH1N1 PR8组可见大量炎症细胞浸润,出现大面积肺水肿;H7N9组表现为大量炎症细胞浸润。结论 H7N9和H1N1 PR8感染BALB/c小鼠均可发病,但发病特点有所差异,H7N9病毒对鼠的适应性比H1N1 PR8差。
Objective To investigate the pathogenesis of A / Shanghai / 4664T (H7N9) and A / Puerto Rico / 8/34 (H1N1) virus-infected BALB / c mice and provide data support for the study of pathogenesis and prevention of H7N9 . Methods BALB / c mice were infected intranasally with the same dose (5 × 103TCID50) of influenza virus, and their changes in body weight, lung index, viral load and lung pathology were analyzed. Results The body weight of mice in H1N1PR8 infection group and H7N9 infection group continued to decrease within 7 days, and H1N1 PR8 group was more obvious than H7N9 group. There was no significant difference in lung index between H7N9 group and PBS group 3 days after infection (P> 0.05) , But the number of H7N9 group was significantly higher than that of PBS group on the 7th day after infection (P <0.05). The lung index of H1N1PR8 group was significantly higher than that of H7N9 group 3 days after infection (P <0.05), but no significant difference was found on the 7th day after infection > 0.05). The viral load of H1N1 PR8 group and H7N9 group increased significantly on the 3rd day after infection, but there was no significant difference between the two groups. On the 7th day, the viral load of both groups decreased, but the viral load of H7N9 group was significantly higher than that of H1N1 PR8 group (P <0.05). The main pathological changes of H1N1 PR8 were inflammatory cell infiltration and edema 3 days after infection, while H7N9 group mainly infiltrated with mild inflammatory cells. A large number of inflammatory cell infiltration occurred in H1N1 PR8 group on day 7 after infection. H7N9 group showed A large number of inflammatory cell infiltration. Conclusion Both H7N9 and H1N1 PR8 can infect BALB / c mice, but the pathogenesis of H7N9 and H1N1 PR8 are different. H7N9 is less adaptive to mice than H1N1 PR8.