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目的前期实验结果显示,压迫超过3 h时,试验大鼠的受压组织明显受损。进一步实验观察不同压迫时间下,SD大鼠血清和组织中的超氧化物歧化酶(SOD)和一氧化氮(NO)结果差异,为寻找压疮患者最佳的翻身时间提供实验室依据。方法将48只SD大鼠分为对照组和实验组,每组8只,对照组未作任何处理,实验组又分为压迫1小时组(即压迫1 h后再解压1 h,再压迫、解压各1 h,依此循环,直至24 h实验结束;简称1 h组,后同)、2小时组(2 h组)、3小时组(3 h组)、4小时组(4 h组)、6小时组(6 h组),共5组,实验组各组的压迫时间与解压时间相等(即缺血和再灌注时间相等),各组缺血和再灌注的总试验时程均为24 h,实验结束后收集各组大鼠的血清和组织,用酶联免疫吸附试验(ELISA)测定血清和组织匀浆液中的SOD和NO的浓度,比较各组SOD和NO的结果差异。结果与对照组相比,3 h组、4 h组和6 h组血清和组织中的SOD均明显降低,而NO明显升高,差异均具有统计学意义(P<0.05),而且随着压迫时间的延长,差异越明显。但1 h组和2 h组的结果差异无统计学意义(P>0.05)。结论压迫超过3 h,大鼠血清和组织中的SOD和NO均发生明显改变,因此,SOD和NO可以作为预测压疮发生的早期无创实验室风险指标。
The purpose of the previous experimental results show that when the pressure over 3 h, the test rats were significantly impaired. Further experiments were conducted to observe the difference of superoxide dismutase (SOD) and nitric oxide (NO) in serum and tissues of SD rats under different oppression time, and to provide a laboratory basis for finding out the optimal turning-over time of pressure ulcer patients. Methods 48 SD rats were divided into control group and experimental group, with 8 rats in each group. The rats in the control group were treated without any treatment. The rats in the experimental group were further divided into 1 hour group (1 h after compression and 1 h decompression, Decompression each 1 h, according to this cycle until 24 h after the end of the experiment; referred to as 1 h group, the same later), 2 hours group (2 h group), 3 hours group (3 h group), 4 hours group (4 h group) , 6 hours group (6 h group), a total of 5 groups, the experimental group of each group of compression time and decompression time equal (ie, ischemia and reperfusion time equal), each group of ischemia and reperfusion test duration were The serum and tissue of each group were collected 24 h after the experiment. The concentrations of SOD and NO in serum and tissue homogenate were determined by enzyme-linked immunosorbent assay (ELISA). The differences of SOD and NO in each group were compared. Results Compared with the control group, the levels of SOD in serum and tissues in 3 h group, 4 h group and 6 h group were significantly decreased, while the NO levels were significantly higher than those in control group (P <0.05) The longer the difference, the more obvious the difference. However, the results of 1 h group and 2 h group showed no significant difference (P> 0.05). CONCLUSIONS: Oxygen and nitric oxide (SOD) and nitric oxide (NO) in serum and tissues of rats significantly change after 3 h of compression. Therefore, SOD and NO can be used as early noninvasive laboratory risk indicators to predict the occurrence of pressure ulcers.