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目的 :初步探讨人类纤溶酶原激活物抑制物 1(plasminogenactivatorinhibitor,PAI 1)启动子区基因多态性与脑血管病的关系及其在脑血管病发病过程中的作用。方法 :通过多聚酶链反应 (polymerasechainreaction ,PCR)技术和发色底物法(ELISA) ,测定 96例脑血管病病人 ,其中脑梗死 (cerebralinfarction ,CI)组 6 5例 ,脑出血 (cerebralhemorrhage ,CH)组 31例和 6 0例对照组的白细胞PAI 1启动子区 4G/ 5G多态性位点的基因型及血浆PAI 1活性。结果 :CI组血浆PAI 1活性明显高于其它二组 ,各组中均以纯合子 4G/ 4G基因型患者的PAI 1血浆活性水平为最高 ,5G/ 5G基因型最低 ,杂合子 4G/ 5G基因型居中 ;4G纯合子基因型与其它二型之间比较差异均有显著意义 ,4G/ 5G与 5G/ 5G基因型之间比较差异无显著意义。CI组 4G/ 4G纯合子型基因型与对照组 (Controls)比较有显著性差异 (P <0 0 5 ) ,CI组基因型与CH组及CH组与对照组基因型比较均无统计学意义 (P >0 0 5 )。女性CI 4G纯合子基因型患者血浆PAI 1活性与同型男性患者比较有显著性差异。结论 :纯合子 4G/ 4G基因型可能是CI发病的危险因素之一 ,4G纯合子个体可能具有较高的CI发病倾向 ,尤其可能与女性CI发病相关
OBJECTIVE: To investigate the relationship between polymorphism of promoter region of human plasminogen activator inhibitor 1 (PAI 1) and cerebrovascular disease and its role in the pathogenesis of cerebrovascular disease. Methods: Ninety-six patients with cerebrovascular disease were determined by polymerase chain reaction (PCR) and chromogenic substrate assay (ELISA), including 65 cases of cerebral infarction (CI), cerebral hemorrhage (CH) The genotype and plasma PAI 1 activity of 4G / 5G polymorphism sites in leukocyte PAI 1 promoter region in 31 patients and 60 control patients were determined. Results: Plasma PAI 1 activity in CI group was significantly higher than that in other two groups. Plasma homozygous 4G / 4G genotype had the highest PAI 1 plasma activity level, lowest 5G / 5G genotype, heterozygous 4G / 5G genotype Centered. There was significant difference between 4G homozygous genotypes and other two types. There was no significant difference between 4G / 5G and 5G / 5G genotypes. The genotypes of 4G / 4G homozygote in CI group were significantly different from those in control group (P <0.05). There was no significant difference in genotype between CI group and CH group and CH group and control group (P> 0 0 5). Plasma PAI 1 activity in female CI 4G homozygous genotypes was significantly different from that in homozygous male patients. Conclusion: The homozygote 4G / 4G genotype may be one of the risk factors for CI. 4G homozygote individuals may have a higher incidence of CI, especially related to the incidence of CI in women