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目的:探讨咖啡酸苯乙酯(CAPE)对结肠癌细胞增殖抑制作用及对c-jun氨基酸末端激酶(JNK)信号通路的影响。方法:体外培养人结肠癌细胞株(HT-29),采用MTT法观察不同浓度的CAPE在不同时间段对HT-29细胞生长的抑制率;不同浓度的CAPE处理HT-29细胞24 h后,用Western blot法检测细胞中JNK及paxillin两种蛋白表达量的变化。结果:4个浓度(2.5,5.0,7.5,10.0μg/L)的CAPE均能明显抑制结肠癌细胞HT-29的增殖,且呈现明显的浓度和时间依赖性(均P<0.01);4个浓度的CAPE均能降低JNK和paxillin蛋白表达水平,且呈明显的浓度依赖性(均P<0.01)。结论:CAPE可在体外抑制人结肠癌细胞HT-29的增殖,机制可能与其降低JNK-paxillin信号通路的活性有关。
Objective: To investigate the inhibitory effect of caffeic acid phenethyl ester (CAPE) on the proliferation of colon cancer cells and the effect on the c-jun amino acid terminal kinase (JNK) signaling pathway. Methods: The human colon cancer cell line (HT-29) was cultured in vitro. MTT assay was used to observe the inhibitory effect of different concentrations of CAPE on the growth of HT-29 cells at different time points. After treated with different concentrations of CAPE for 24 h, Western blot was used to detect the expression of JNK and paxillin in the cells. Results: CAPE at four concentrations (2.5, 5.0, 7.5 and 10.0 μg / L) significantly inhibited the proliferation of HT-29 colon carcinoma cells in a concentration- and time-dependent manner (all P <0.01) The concentration of CAPE reduced the expression of JNK and paxillin protein in a concentration-dependent manner (all P <0.01). CONCLUSION: CAPE can inhibit the proliferation of human colon cancer HT-29 cells in vitro, which may be related to the decrease of the activity of JNK-paxillin signaling pathway.