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许多学者基于巨噬细胞的贴壁特性曾使用多种方法从外周血分离人的巨噬细胞。但目前使用的一些方法尚有不足之处,可归纳为以下三点: 1.细胞的收获量和活力往往不稳定; 2.或者需要预先用小牛血清处理塑料物; 3.或者需要使用以前贴壁的细胞株的微量渗出物包被塑料物。 1978年Rinehart等虽用利多卡因(Lidoeathe)从未经处理过的塑料表面分离过人的单核细胞,但用他这种方法收获的细胞量又少又不稳定,细胞的活力也较差。本文作者所使用的低pH值利多卡因改良法可以经常产生功能好的人单核细胞,并与下列几种贴壁分离法作了比较:(1)用橡皮头(rubber policeman)进行手工刮除法;(2)用微量渗出物包被塑料物;(3)
Many scholars have used a variety of methods to separate human macrophages from peripheral blood based on their macrophage adherence properties. However, there are still some shortcomings of the current methods, can be summarized as the following three points: 1. Cell harvest and vitality are often unstable; 2. Or need to pre-treated plastic with bovine serum; 3. Or need to use before Micro-exudates of adherent cell lines are coated with plastic. In 1978, Rinehart et al. Used Lidoeathe to separate supernumerary mononuclear cells from untreated plastic surfaces. However, the amount of cells harvested by this method was less and less stable, and cell viability was poor . The low pH lidocaine modification used by the authors of the present invention often produces well-functioning human monocytic cells and compares them with the following adherent separation methods: (1) hand-scraping with rubber policeman Division; (2) plastic coated with trace exudate; (3)