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目的探讨蓝百合提取物(Agapanthus africanus L extracts,AE)的镇咳效果及其初步作用机制。方法采用雾氨、二氧化硫诱导小鼠咳嗽实验以及辣椒素诱导豚鼠咳嗽实验来评价AE镇咳的效果;采用辣椒素诱导鼠肺泡及血清释放C-纤维P物质,观察AE外周镇咳作用途径。结果与对照组相比,高剂量的AE(400 mg·kg-1)不但可有效地延长雾氨以及二氧化硫诱导小鼠咳嗽的潜伏期(P<0.01),也可有效减少咳嗽次数(P<0.05),且各剂量间有较好的量效关系;高剂量的AE可显著地抑制辣椒素诱导的豚鼠咳嗽次数(P<0.01)。与对模型相比,AE对辣椒素诱导豚鼠肺泡及血清C-纤维P物质的释放有显著的抑制作用(P<0.05)。结论 AE具有较好的镇咳作用,其效果可能与AE抑制C-纤维P物质的释放有关。
Objective To investigate the antitussive effect and its primary mechanism of Agapanthus africanus L extracts (AE). Methods The effect of antitussive on AE was evaluated by mist ammonia, sulfur dioxide-induced mouse cough and capsaicin-induced guinea pig cough. The capsaicin was used to induce the release of C-fiber substance P from the alveoli and serum of the rats. Results Compared with the control group, high dose of AE (400 mg · kg-1) not only prolonged the latent period of cough induced by mist ammonia and sulfur dioxide (P <0.01), but also reduced the frequency of cough (P <0.05 ), And there was a good dose-response relationship among all doses. A high dose of AE significantly inhibited capsaicin-induced guinea pig cough frequency (P <0.01). Compared with the model, AE had a significant inhibitory effect on the release of capsaicin-induced alveolar and C-fiber P in guinea pigs (P <0.05). Conclusion AE has a good antitussive effect, and its effect may be related to the inhibition of C-fiber release of substance P by AE.