硬皮病属于系统性自体免疫疾病,主要表现为皮肤和内脏器官的纤维化与细胞外基质成分的过度合成。为探讨本病硬化性成纤维细胞内紊乱的分子基础,作者通过连续细胞核转录和印迹分析,比较了本病患者与正常人成纤维细胞热休克(heat-shock)蛋白(hsp)70基因表达。活检患者病灶皮肤获得硬化性成纤维细胞,取无风湿病史、年龄、性别及部位与患者相匹配的正常皮肤成纤维细胞作对照,作 Scleroderma is a systemic autoimmune disease, mainly manifested as excessive synthesis of fibrosis of the skin and internal organs and extracellular matrix components. To explore the molecular basis of this disorder in sclerosing fibroblasts, the authors compared the expression of heat shock protein 70 (hsp70) in patients with normal human fibroblasts by serial nuclear transcripts and Western blot analysis. Biopsy specimens of patients with sclerodermal fibroblasts obtained, taking no history of rheumatism, age, gender and location of the patient matched normal skin fibroblasts as a control for