目的建立HPLC法测定小儿止痒胶囊中防风所含升麻素苷和5-O-甲基维斯阿米醇苷含量的方法。方法采用D iamonsil(TM)钻石C18(250 mm×4.6 mm,5μm),甲醇-水(40∶60)为流动相;流速为1.0 mL/m in,检测波长为254 nm。结果升麻素苷在0.388 8~0.907 2μg范围内线性良好(r=0.999 9),5-O-甲基维斯阿米醇苷在0.127 2~0.763 2μg范围内线性良好(r=0.999 6)。升麻素苷平均回收率为102.3%,RSD为2.59%(n=5);5-O-甲基维斯阿米醇苷平均回收率为98.1%,RSD为1.53%(n=5)。结论本方法简便,结果准确。 Objective To establish a HPLC method for the determination of the content of colicin and 5-O-methylstildemidoside in children’s antipruritic capsules. Methods Diamonsil(TM) Diamond C18 (250 mm×4.6 mm, 5 μm), methanol-water (40:60) was used as the mobile phase, the flow rate was 1.0 mL/min, and the detection wavelength was 254 nm. RESULTS: The linearity of cocinin was good in the range of 0.388 8 to 0.907 2 μg (r=0.999 9), and the linearity of 5-O-methylvasimidide was good in the range of 0.127 2 to 0.763 2 μg (r=0.999 6). . The average recovery rate of coprofen was 102.3%, and the RSD was 2.59% (n=5). The average recovery rate of 5-O-methylvasimidide was 98.1%, and the RSD was 1.53% (n=5). Conclusion This method is simple and accurate.