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目的 使用传统的巯基显色剂Ellman试剂测量并比较分析人类不同年龄段的透明及各类型白内障晶状体样本中非蛋白巯基(游离巯基)、蛋白巯基和蛋白结合巯基的含量,并分析其与人类年龄相关性白内障形成的关系。方法44个人类晶体按年龄大小被分为5组,其中10个白内障晶体被分成2组(皮质性和核性)。用Ellman试剂测量以上样 品的晶体非蛋白巯基、总蛋白巯基和总蛋白结合巯基含量。 结果 皮质性白内障晶状体的非蛋白巯基含量显著高于核性白内障晶状体(P<0.01);而在蛋白巯基和蛋白结合巯基中,两者则无显著差别(P>0.05)。非蛋白巯基含量从胚胎期到年龄相关性白内障发生逐渐降低,各年龄组均显著小于前一年龄组(P<0.01);蛋白巯基含量在第5组显著高于第1、2、3、4组(P<0.05),其余各组间无显著区别(P>0.05);蛋白结合巯基含量除第3、4组和第4、5组间无显著区别外(P>0.05),其余组均显著低于较小年龄组(P<0.01)。 结论 人类年龄相关性白内障的形成过程中,晶状体蛋白分子间巯基的氧化并不像在其它实验性白内障模型中那样起决定作用。
Objective To measure and compare the contents of non-protein sulfhydryl (free sulfhydryl), sulfhydryl and protein sulfhydryl groups in transparent and various types of cataract lenses of human at different ages using traditional reagent thiol reagent Ellman’s reagent, and to analyze its relationship with human age Related cataract formation. Methods Forty-four human crystals were divided into five groups according to their age. Ten of the cataract crystals were divided into two groups (cortex and nucleus). Crystalline non-protein sulfhydryl groups, total protein sulfhydryl groups and total protein bound sulfhydryl groups of the above samples were measured with Ellman’s reagent. Results The content of non-protein sulfhydryl group in cataractous lens was significantly higher than that in nuclear lens (P <0.01). There was no significant difference between sulfhydryl group and protein sulfhydryl group (P> 0.05). The content of non-protein sulfhydryl group decreased gradually from embryonic stage to age-related cataract, and was significantly lower in all age groups than in the previous age group (P <0.01). The content of sulfhydryl group in group 5 was significantly higher than that in groups 1, 2, 3, (P <0.05). There was no significant difference between the other groups (P> 0.05). The contents of protein-bound sulfhydryl groups were not significantly different between groups 3 and 4 and groups 4 and 5 Significantly lower than the younger age group (P <0.01). Conclusion Oxidation of sulfhydryl groups between lens protein molecules is not as decisive as other experimental cataract models in the development of human age-related cataract.