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目的:探讨血管紧张素转换酶2(ACE2)基因的多态性与皖北汉族人冠状动脉粥样硬化性心脏病(冠心病,CHD)的关系。方法:采用聚合酶链反应和限制性片段长度多态性(PCR-RFLP)的方法,检测120例CHD患者与109例对照人群(排除CHD患者及健康体检者)的ACE2基因,并进行组间对照研究ACE2基因的多态性与CHD的相关性。结果:在男性CHD患者中,G等位基因的频率明显高于对照组(分别为77.6%、60.7%,P<0.05);在女性CHD患者中,携带G等位基因的基因型GG型、AG型的分布频率略高于对照组(分别为43.2%、29.5%和32.1%、28.3%),差别无统计学意义。结论:ACE2基因的多态性与皖北男性汉族人群CHD的发病可能存在相关性,携带G等位基因的男性人群发生CHD的危险性相对较大。
Objective: To investigate the relationship between angiotensin converting enzyme 2 (ACE2) gene polymorphism and coronary heart disease (CHD) in Han population in North Anhui. Methods: Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method was used to detect ACE2 gene in 120 CHD patients and 109 control subjects (excluding CHD patients and healthy subjects) Control study of ACE2 gene polymorphism and CHD correlation. Results: The frequency of G allele in CHD patients was significantly higher than that in the control group (77.6% and 60.7%, respectively, P <0.05). In genotypes GG with G allele in women with CHD, The distribution frequency of AG type was slightly higher than that of the control group (43.2%, 29.5% and 32.1%, 28.3%, respectively), but the difference was not statistically significant. Conclusion: The polymorphism of ACE2 gene may be related to the incidence of CHD in Han Han population of northern Anhui Province. The risk of CHD in male population with G allele is relatively high.