论文部分内容阅读
近年来,许多作者对血小板计数(PC)方法学进行子不少研究。多数人认为溶血汉优于不溶血法,而溶血法又倾向于草酸铵法及盐酸普鲁卡因法。本文采用一人、双份、两组的采血及计数法,以同种型号的显微镜、计数板、Hb吸管及同一试剂对1％草酸法和2％盐酸普鲁卡因法进行了61人份244次的比较及两法的改良。现介绍如下: 一、材料与方法 1.稀释液 (1)2％盐酸普鲁卡因溶液:取医用盐酸普鲁卡因(中国医药工业公司上海第八制药厂生产,批号680326—1)2.00 g,加蒸馏水50 ml,溶解后定容到100 ml,4℃冰箱保存。 (2)2％普鲁卡因改良液:在2％普鲁卡因溶液中加入0.1 ml甲醛溶液,混匀,备用。
In recent years, many authors have done a lot of research on platelet counting (PC) methodology. Most people think hemolytic better than the non-hemolytic method, and the hemolysis and tend to ammonium oxalate method and procaine hydrochloride. In this paper, one person, two copies, two sets of blood collection and counting method, with the same type of microscope, counting plate, Hb pipette and the same reagent 1% oxalic acid and 2% procaine hydrochloride method for the people of 244 Comparison and improvement of two laws. Now introduced as follows: First, the material and methods 1. Dilution (1) 2% procaine hydrochloride solution: Take the medical procaine hydrochloride (China Pharmaceutical Industry Corporation Shanghai No. 8 Pharmaceutical Factory production, batch number 680326-1) 2.00 g, add distilled water 50 ml, dissolve after volume to 100 ml, 4 ℃ refrigerator. (2) 2% procaine modification solution: Add 0.1 ml formaldehyde solution to 2% procaine solution, mix and reserve.