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目的对连云港市2010年手足口病例标本进行病原学检测,了解手足口病的感染情况,分析检测结果,为手足口病的预防和临床诊断提供科学依据。方法采用Real Time RT-PCR方法对手足口病患者咽拭子、肛拭子标本进行肠道病毒(EV)、肠道病毒71型(EV71)和柯萨奇病毒16型(CoxA16)特异性核酸的检测。结果 475例患者标本中EV71阳性率27.8%(132例),CoxA16阳性率20.0%(95例),其他肠道病毒阳性率7.8%(37例);全年的感染高峰是4～6月份,占全年的39.58%;发病年龄以低年龄组为主,0~3岁感染率为77.68%。结论手足口病发病有明显的时间和年龄差异,连云港市手足口病病原以EV71和CoxA16为主,重症病例的病原以EV71为主,荧光定量RT-PCR可快速诊断手足口病病原。
Objective To detect the pathogen of hand, foot and mouth swab specimens in Lianyungang City in 2010 to understand the infection of hand, foot and mouth disease, and to analyze the test results so as to provide a scientific basis for prevention and clinical diagnosis of hand foot and mouth disease. Methods Real-time RT-PCR was used to detect the presence of enterovirus (EV), enterovirus 71 (EV71) and CoxA16 specific nucleic acids in throat and rectal swabs of HFMD patients. The test. Results The positive rate of EV71 was 27.8% (132 cases), the positive rate of CoxA16 was 20.0% (95 cases) and the positive rate of other enterovirus was 7.8% (37 cases) in 475 cases. The annual infection peak was from April to June, Accounting for 39.58% of the whole year; the age of onset was mainly in the lower age group, and the infection rate of 0 to 3 years old was 77.68%. Conclusions The incidence of HFMD is obviously different with age and age. EV71 and CoxA16 are the main pathogens of HFMD in Lianyungang City. EV71 is the most common pathogen of severe HFMD. Fluorescent quantitative RT-PCR can be used to diagnose HFMD.