Due to the high efficiency of ammonia nitrogen degradation, a novel Candida sp. strain N6 was applied for wastewater treatment by using immobilization technology. Immobilization conditions and corresponding ammonia removal capacity of immobilized N6 beads were investigated.The immobilized N6 beads were applied to degrade simulated ammonia nitrogen wastewater. Results showed that the optimum formula of immobilized materials was 9% polyvinyl alcohol, 1.5% sodium alginate, and 2% calcium chloride, under which the ammonia nitrogen removal rate of the immobilized N6 beads reached 97.97%. The following immobilization conditions were observed to be optimal: the immobilization time was 24 h, the inoculum of N6 was 3%, and the pH was 8.The immobilized N6 beads exhibited excellent ammonianitrogen degradation ability in SBRs. The ammonia nitrogen removal rate was stable at 95%-99% in the SBRs. The results indicated that immobilized N6 beads possess good application prospects in the treatment of ammonia nitrogen wastewater.