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目的研究非酒精性脂肪肝模型大鼠的血清C-反应蛋白(CRP)水平变化,探讨其与肝功能之间的关系。方法实验大鼠分为模型组(HF)、模型恢复组(FC)和对照组(NC)。NC组采用基础饲料喂养,HF组采用高脂饲料喂养,FC组高脂喂养8周后改基础饲料喂养。模型建立后取大鼠空腹血测定血清肝酶系、血脂及CRP水平,肝脏标本作组织病理学检查。结果高脂饲料喂养8周后,大鼠肝脏出现典型脂肪变性及炎症细胞浸润;12周后有重度脂肪变性伴大量炎症细胞浸润。HF组血清肝酶系(ALT、AST)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)水平均较NC组升高,血清CRP水平(0.43±0.14)mg/L亦高于NC组(0.14±0.08)mg/L。采取饮食控制的FC组肝脏病变程度减轻,ALT、AST、TC和HDL-C水平下降,CRP水平亦较HF组降低(0.21±0.14)mg/L。相关性分析显示脂肪肝大鼠的血清CRP水平与ALT水平正相关(r=0.56,P<0.05)。结论非酒精脂肪肝模型大鼠血清CRP水平升高,并与肝功能指标(ALT水平)正相关,CRP可能是非酒精性脂肪肝的预测因素之一。
Objective To study the changes of serum C-reactive protein (CRP) in non-alcoholic fatty liver model rats and to explore its relationship with liver function. Methods Experimental rats were divided into model group (HF), model recovery group (FC) and control group (NC). The rats in NC group were fed with basal diet, the HF group were fed with high fat diet, and the FC group were fed with basal diet after 8 weeks of high fat diet. After establishment of the model, the fasting blood of rats was used to measure the levels of serum liver enzymes, blood lipids and CRP, and the liver specimens were examined for histopathology. Results After fed with high-fat diet for 8 weeks, typical steatosis and inflammatory cell infiltration occurred in the liver of rats. Severe steatosis and infiltration of inflammatory cells were observed after 12 weeks. The levels of serum ALT, TC and HDL-C in HF group were significantly higher than those in NC group (0.43 ± 0.14) mg / L NC group (0.14 ± 0.08) mg / L. The degree of hepatic lesion, the level of ALT, AST, TC and HDL-C in the diet control FC group decreased and the level of CRP in the FC group decreased (0.21 ± 0.14) mg / L compared with the HF group. Correlation analysis showed that serum CRP level in ALF was positively correlated with ALT (r = 0.56, P <0.05). Conclusion The serum level of CRP in non-alcoholic fatty liver model rats is increased, which is positively correlated with liver function index (ALT level). CRP may be one of the predictors of nonalcoholic fatty liver disease.