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目的:观察贝那普利和全反式维甲酸对早期糖尿病肾病大鼠的血生化和肾组织的影响。方法:成年雄性Wistar大鼠,分为对照组(NC)、DN模型组(DN)、DN+贝那普利组(ACEI)和DN+ATRA组(ATRA)。ACEI组给予贝那普利灌胃,ARTA组给予ATRA灌胃,NC和DN组以等量蒸馏水灌胃。饲养4周后观察大鼠生理生化指标的变化及肾脏组织病理学变化。结果:DN组、ACEI组和ATRA组大鼠体重增长缓慢,DN组、ACEI组和ATRA组大鼠血糖较NC显著升高(P<0.01),DN组肾重、肾脏肥大指数及尿UAlb/UCr均显著高于DM组,ACEI组和ATRA组较DN组则明显下降(P<0.01),差异均有统计学意义,而ACEI组和ATRA组上述指标无明显差异。肾脏病理显示DN组肾小球体积明显增大,细胞肥大,系膜基质轻度增生,基底膜略增厚,ACEI组和ATRA组上述改变较DN组为轻,肾小球体积有所缩小,系膜基质增生不明显,而ACEI组和ATRA组则无明显差异。结论:贝那普利和ATRA对早期糖尿病肾病均有治疗作用,为DN的早期防治提供线索和依据。但二者之间的比较暂无明显差异。
OBJECTIVE: To observe the effects of benazepril and all-trans retinoic acid on blood biochemistry and renal tissue in early diabetic nephropathy rats. Methods: Adult male Wistar rats were divided into control group (NC), DN model group (DN), DN + benazepril group (ACEI) and DN + ATRA group (ATRA). ACEI group given benazepril gavage, ARTA group given ATRA gavage, NC and DN group with the same amount of distilled water gavage. Four weeks after feeding, the changes of physiological and biochemical indexes and pathological changes of kidney were observed. Results: The body weight of rats in DN group, ACEI group and ATRA group increased slowly. The blood glucose of DN group, ACEI group and ATRA group were significantly higher than that of NC group (P <0.01). The renal weight, renal hypertrophy index and urinary UAlb / UCr were significantly higher than the DM group, ACEI group and ATRA group was significantly decreased compared with DN group (P <0.01), the difference was statistically significant, while ACEI group and ATRA group of the above indicators no significant difference. Kidney pathology showed that glomerular volume increased significantly in DN group, cell hypertrophy, mild hyperplasia of mesangial matrix, basement membrane slightly thickening, ACEI group and ATRA group compared with DN group, the change was light, glomerular volume was reduced, Mesangial stromal hyperplasia was not obvious, while there was no significant difference between ACEI group and ATRA group. Conclusion: Both benazepril and ATRA have therapeutic effects on early diabetic nephropathy, providing clues and basis for the early prevention and treatment of DN. However, there is no obvious difference between the two.