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目的 研究外周血T淋巴细胞核仁区嗜银蛋白(AgNORs)染色对传染性非典型肺炎(SARS)的临床意义。方法 确诊SARS患者14例非SARS肺炎和正常对照各8例,抽取外周血,PHA刺激外周血T淋巴细胞培养,KL型肿瘤免疫图像分析系统分析AgNORs区面积与细胞核仁区的百分比(I.S％),计算活化T细胞的百分率。结果I.S％和活化率分别为:8名正常对照组为7.38％±0.75％和63.64％±11.65％,8例非SARS肺炎患者为8.98％±0.91％和57.14％±12.49％,14例SARS患者为4.13％±3.24％和10.64％±8.30％,出院后1个月随访患者5例为10.80％±0.33％和65.89％±12.13％。病情危重的患者活化细胞率为0,提示T细胞功能几乎完全丧失;处于恢复期的患者T细胞免疫功能仍未恢复到正常;治愈出院1月后随访患者的T细胞免疫功能恢复到高于正常水平。结论 AgNORs测定对SARS患者的诊断、病情监测、出院和康复评价有一定的指导意义。
Objective To study the clinical significance of staining for nucleolar amyloid protein (AgNORs) in peripheral blood T lymphocytes for SARS. Methods Fourteen non-SARS pneumonia patients and 14 healthy controls were included in the study. Peripheral blood and PHA-stimulated peripheral blood T lymphocytes were collected from 14 patients with SARS. The percentages of AgNORs area and nucleolar area (IS%) were analyzed by KL immunohistochemical analysis system. , Calculate the percentage of activated T cells. Results The percentages of IS% and activation were respectively 7.38% ± 0.75% and 63.64% ± 11.65% in 8 normal controls, 8.98% ± 0.91% and 57.14% ± 12.49% in 8 non-SARS pneumonia patients, and 14 patients with SARS 4.13% ± 3.24% and 10.64% ± 8.30% respectively. Among the 5 cases who were followed up one month after discharge, they were 10.80% ± 0.33% and 65.89% ± 12.13%. T cell immune function was not recovered to normal in patients with convalescent T cell immune function returned to normal after more than normal Level. Conclusion The AgNORs determination has certain guiding significance for the diagnosis, condition monitoring, discharge and rehabilitation evaluation of SARS patients.