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研究甲氨蝶呤(MTX)在石墨烯修饰玻碳电极上的电化学行为,探讨MTX与溶菌酶(LYSO)的相互作用及其作用机理。结果发现,在pH 4.5的HAc-NaAc缓冲溶液中,石墨烯修饰电极对MTX的电化学氧化具有明显的催化作用,氧化峰电流相对于在裸玻碳电极上增加了5倍。线性范围为0.05~3.0μmol/L,检出限(S/N=3)为0.02μmol/L。对0.8μmol/L的MTX11次平行测定,RSD为3.5%。当LYSO加入MTX溶液后,其峰电流降低。紫外光谱有红移增色效应。MTX与LYSO结合比为1∶1,结合常数为4.9×105L/mol。方法可用于MTX片药物的检测及与蛋白相互作用的研究。
To investigate the electrochemical behavior of methotrexate (MTX) on graphene modified glassy carbon electrode and to explore the interaction between MTX and lysozyme (LYSO) and its mechanism of action. The results showed that the graphene modified electrode had a significant catalytic effect on the electrochemical oxidation of MTX in pH 4.5 HAc-NaAc buffer solution, and the oxidation peak current increased by 5 times compared with that on bare glassy carbon electrode. The linear range was 0.05 ~ 3.0μmol / L, and the detection limit (S / N = 3) was 0.02μmol / L. The RSD was 3.5% for a parallel assay of 0.8 μmol / L MTX 11 times. When LYSO was added to MTX solution, the peak current decreased. UV spectral red-shift enhancement effect. The binding ratio of MTX to LYSO was 1: 1, and the binding constant was 4.9 × 10 5 L / mol. The method can be used for the detection of MTX tablets and its interaction with proteins.