本文对常规随机引物标记基因探针法进行了改进，排除了已标记载体ＤＮＡ片段在核酸分子杂交反应中的影响，证明了载有特异ｃＤＮＡ片段质粒直接用于标记探针的可行性，建立了一种快捷、简便、经济的基因探针制备方法，并为特异ＤＮＡ片段酶切、回收困难质粒的应用提供了一条可行之路 In this paper, the conventional random primer-labeled gene probe method was improved to exclude the effect of the labeled vector DNA fragments in the hybridization reaction of nucleic acid molecules. It was proved that the plasmid carrying the specific cDNA fragment was directly used for the labeling of probes. A quick, simple and economical method of gene probe preparation, and provides a feasible way for the application of specific plasmid for DNA fragment digestion and recovery