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抗人 A 血型杂交瘤15B_4细胞在 CelliGen 细胞培养罐中进行连续灌注培养,细胞密度达1.6×10~7/ml。每天灌注新培养液量由1000ml 增到2300ml,第14天后又逐日减少至1000ml,死细胞数随着转速的增加和灌注量的减少而增多。血凝效价呈梯度上升,滴度达到2~(14),相当于腹水效价。培养6天 IgM 产量为0.2克/升/天,10～18天波动在1.45～1.8克/升/天。15B_4细胞代谢与葡萄糖、乳酸有关,与氨无关。CelliGen 自控效果较好,适用于杂交瘤细胞的高密度培养。搅拌速度在120～150r/min 对细胞产生的剪切力有明显地破坏作用。溶氧控制系统有待改进。
Anti-human A blood type hybridoma 15B_4 cells were continuously perfusion cultured in CelliGen cell culture tank with a cell density of 1.6 × 10 ~ 7 / ml. The volume of new culture fluid per day increased from 1000ml to 2300ml, and then decreased to 1000ml day after day 14, the number of dead cells increased with the increase of speed and the decrease of perfusion. Hemagglutination titer showed a gradient increase, titer reached 2 ~ (14), equivalent to ascites titer. IgM production at 6 days was 0.2 g / L / day and fluctuated between 1.45 and 1.8 g / L / day in 10-18 days. 15B_4 cell metabolism and glucose, lactate, has nothing to do with ammonia. CelliGen self-controlled effect is better, suitable for high-density hybridoma cell culture. Stirring speed at 120 ~ 150r / min on the shear stress generated by the cells have a significant role in the destruction. Dissolved oxygen control system needs improvement.