用多管微电极技术，在麻醉、自主呼吸的大鼠旁巨细胞外侧核尾半侧（ｃＰＧＣＬ）观察了微电泳乙酰胆碱（ＡＣｈ）、甘氨酸（Ｇｌｙ）及其拮抗剂阿托品、士的宁对神经元自发放电的效应及阿托品对ＡＣｈ、士的宁对甘氨酸效应的影响。发现ＡＣｈ可使大多数被测试神经元（７９．６％）兴奋，少数（２０．４％）无反应；甘氨酸可使大多数被测试神经元（９１．５％）抑制，少数（８．５％）无反应；阿托品可使神经元兴奋（占被测试神经元的８％）、抑制（４８％）和无反应（４４％）；士的宁可引起神经元兴奋（２１．９％）、抑制（６．３％）和无反应（７１．８％）。ＡＣｈ的兴奋效应，甘氨酸和阿托品的抑制效应均呈量效依赖关系。阿托品可部分或完全阻断大多数被测试神经元（８０．３％）对ＡＣｈ的兴奋反应；士的宁可阻断大多数被测试神经元（９２．６％）对甘氨酸的抑制反应。以上结果提示，ｃＰＧＣＬ区存在着内源性ＡＣｈ和甘氨酸，某些神经元上存在着Ｍ受体及甘氨酸受体 Microelectrophoresis of acetylcholine (ACh), glycine (Gly) and its antagonists atropine and strychnine on nerve of the nerve of rats were observed by multi-tube microelectrode technique in the cPGCL of paramaxillary nucleus next to anesthetized and spontaneously breathing rats Effect of spontaneous discharge and the effect of atropine on glycine effect of ACh and strychnine. Most of the tested neurons (79.6%) were found to be excited by ACh, and a few (20.4%) were unresponsive. Glycine inhibited most of the tested neurons (91.5% %) Showed no response; atropine excitated neurons (8% of tested neurons), inhibition (48%) and no response (44%); strychnine rather induced neuronal excitation (21.9% (6.3%) and no response (71.8%). The excitatory effect of ACh, the inhibitory effect of glycine and atropine showed a dose-dependent relationship. Atropine partially or completely blocked most of the tested neurons (80.3%) on the ACh excitatory response; strychnine rather blocked the most tested neurons (92.6%) inhibition of glycine response. The above results suggest that there are endogenous ACh and glycine in cPGCL region and M receptor and glycine receptor on some neurons