【主干知识】1.了解植物组织培养技术在生产中的应用;2.熟悉植物组织培养或花药培养的基本过程;3.了解影响植物组织培养或花药培养的各种因素;4.归纳植物组织培养与花药离体培养的区别;5.掌握培养基制备、外植体消毒、接种、培养、移栽以及栽培等基本操作技术。 【Main knowledge】 1. To understand the application of plant tissue culture technology in production; 2. To be familiar with the basic process of plant tissue culture or anther culture; 3. To understand various factors that affect plant tissue culture or anther culture; 4. Induction of plant tissue The difference between culture and anther culture in vitro; 5. Master the basic operating techniques such as medium preparation, disinfection of explants, inoculation, cultivation, transplanting and cultivation.