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取6～12个月雌兔分别关在有空调及12小时光照的室内,用混合饲料及水喂养两周后供实验用。卵巢制备及灌流装置可参考Janson报道(1982),灌流液用Earle氏液加谷氨酸等(GIBCO实验室)介质199pH为7.4,总循环灌流液量为80ml,在开始循环,灌流装置已平衡条件下做卵巢手术。卵巢开始灌流后1小时每对卵巢中的一个加LH0.1或1μg/ml,另一卵巢作对照,卵巢放入灌流装置前和后各1小时及其后每隔2小时取2 ml灌流液作试样。本试验共用9对卵巢灌流,每15～30分钟检查一次卵泡形状及大小,以便记录排卵情况,一般情
Take 6 to 12 months old female rabbits were housed in air-conditioned and 12-hour light indoors, with mixed feed and water for two weeks for experimental use. Ovarian preparation and perfusion device can refer to Janson reported (1982), perfusion fluid with Earle’s solution plus glutamic acid (GIBCO laboratory) medium 199pH was 7.4, the total circulating fluid volume of 80ml, at the beginning of circulation, perfusion device has been balanced Under the conditions of ovarian surgery. 1 hour after ovariectomy, one ovary in each ovary plus LH0.1 or 1μg / ml, the other ovary as a control, the ovary into the perfusion device before and after each 1 hour and every 2 hours thereafter take 2 ml perfusate Make a sample. This experiment shared 9 pairs of ovarian perfusion, check the shape and size of follicles every 15 to 30 minutes in order to record ovulation conditions, the general situation