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目的:研究莪术残油(提取榄香烯后的残余莪术油)中两种指标性成分在大鼠体内的各组织分布。方法:采取大鼠灌胃给药,运用毛细管气相色谱法采用程序升温条件,以α-香附酮为内标物同时测定莪术残油中指标性成分莪术醇、莪术二酮在大鼠各个组织中的药物浓度。结果:莪术醇在0.002628～0.2102mg·mL-1范围内呈现良好的线性关系,莪术二酮在0.0004552～0.03642mg·mL-1范围内呈现良好的线性关系,精密度、稳定性及融冻次数考察均符合要求,测定方法的平均绝对回收率莪术醇为83.6%～88.7%,莪术二酮为80.6%～85.0%;内标物α-香附酮的平均绝对回收率为82.6%～85.8%;平均方法回收率莪术醇为71.9%～76.1%,莪术二酮为69.7%～72.8%,均符合有关要求。通过各组织中指标性成分含量分析,各组织中均含有两种指标性成分。结论:莪术残油口服给药对于大鼠的组织作用靶点可能是脑、脾和肾。
Objective: To study the distribution of two kinds of index components in the Curcuma oil residue (residual Curcuma oil after the extraction of elemene) in rats. Methods: The rats were given gavage by capillary gas chromatography with temperature programmed conditions, α-ketones as an internal standard substance for the simultaneous determination of Curcuma residue oil Curcumol, Curdione in various tissues of rats In the drug concentration. Results: Curcumol showed a good linearity in the range of 0.002628 ~ 0.2102 mg · mL-1. Curcumaldione showed a good linearity in the range of 0.0004552 ~ 0.03642 mg · mL-1, the precision, stability and the number of freeze-thaw cycles The average absolute recovery was 83.6% ~ 88.7% for curcumol and 80.6% ~ 85.0% for curdione. The average absolute recoveries of α-ketoconazole was 82.6% ~ 85.8% ; The average recovery rate of curcumol was 71.9% -76.1%, and curdione was 69.7% -72.8%, all of which met the relevant requirements. Through the analysis of the index content of each organization, each organization contains two index components. Conclusion: The effects of oral administration of Curcuma residue on the tissue of rats may be brain, spleen and kidney.