1引言测定蛋白质的浓度是生物学，医学和化学等科学领域的基本要求。在测定蛋白质含量的众多方法中，较常用的有h吁法，BCA法，Bradfni法，但这几种方法的缺点是灵敏度不高，它们的检测限大约在l－5ngL范围内。首素红S是一种天然阴离子染料，它的分光光度法测定蛋白质已有报道 1 Introduction Determining the concentration of a protein is a basic requirement in the fields of biology, medicine and chemistry. Of the many methods used to determine protein content, there are more commonly used methods, BCA method, Bradfni method, but the disadvantage of these methods is the sensitivity is not high, their detection limit is about l-5ngL range. The first prime red S is a natural anionic dye, its spectrophotometric determination of protein has been reported