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作者对冷冻精液的四种复温方法及两种保护剂分别进行了202例和194例次的对比观察。结果表明:冷冻的复温效果受复温方法影响.快复温比慢复温效果好,37℃水浴和48℃水浴复温效果明显优于室温(22℃)空气中和孵箱(37℃)内放置(P<0.001),并结合观察结果,对快速复温及效果良好的机制进行了讨论。甘油和甘油-卵黄-柠檬酸钠复合剂两种冻精复温后,精子存活率十分接近(P>0.05).表明两种保护剂对精子的保护效能大致相同.
The author of the frozen semen four rewarming methods and two protective agents were 202 cases and 194 cases were observed. The results showed that the rewarming effect of freezing was affected by the rewarming method, the effect of rewarming was better than that of slow rewarming, the rewarming effect of 37 ℃ water bath and 48 ℃ water bath was better than that of room temperature (22 ℃) in air and incubator ) (P <0.001), and combined with the observations, the mechanisms of rapid rewarming and well-acting were discussed. The survival rate of sperm was close to that of glycerol and glycerol-yolk-sodium citrate complex after spermethrin rewarming (P> 0.05), indicating that the two protective agents have similar protective effect on sperm.