以高代自交系“东农11537”番茄幼苗为试验材料,通过叶面喷施不同浓度的脱落酸,在筛选出适宜浓度为200μL/LABA的基础上,进一步探讨外源喷施ABA对番茄幼苗抗冷性的影响。结果表明,在200μL/L的ABA预处理番茄幼苗后,随冷处理时间延长,叶片内脯氨酸、可溶性糖和可溶性蛋白含量均逐渐升高,在同一时期的抗氧化酶活性和叶绿素含量高于CK,而MDA含量低于CK。冷胁迫下两组叶片中抗冷相关基因CBF1、SLCZFP1、SLCMYB1、LENLP4的表达量都出现上升趋势,其中SLCMYB1、LENLP4两个基因在ABA预处理后表达量上升显著高于CK,经ABA预处理的幼苗能够促进冷胁迫相关基因的表达并提高其抗冷性。 Taking the high-generation inbred lines Dongnong 11537 tomato seedlings as test materials, different concentrations of abscisic acid were sprayed on the foliar surface. Based on the screening of the suitable concentration of 200μL / LABA, ABA Effect of chilling tolerance on tomato seedlings. The results showed that the contents of proline, soluble sugar and soluble protein in leaves increased gradually with the prolongation of cold treatment after 200μL / L ABA pretreatment of tomato seedlings, and the antioxidant enzyme activities and chlorophyll contents were higher in the same period CK, while MDA content is lower than CK. Under cold stress, the expressions of CBF1, SLCZFP1, SLCMYB1 and LENLP4 in both leaves showed an upward trend. The expression of SLCMYB1 and LENLP4 in ABA pretreated groups was significantly higher than CK, Of seedlings can promote the expression of cold stress related genes and improve their cold resistance.