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以山东寿光地区感染辣椒轻斑驳花叶病毒的辣椒为试材,采用试剂盒提取感病辣椒的总RNA并进一步反转录成cDNA,参照已报道的PMMoV检测引物合成特异性引物PMMoV-F和PMMoV-R;以获得的cDNA为模板进行PCR扩增,研究了辣椒轻斑驳花叶病毒寿光分离物。结果表明:得到分子量约是576bp的条带,纯化后进行基因测序;通过测序结果分析比对可知,PMMoV寿光分离物序列与诸多地区的分离物同源性较高,可达到99%~100%。
In this study, the capsicum was infected with pepper light mottle mosaic virus in Shouguang, Shandong Province. The total RNA was extracted from the infected capsicum with a kit and further transcribed into cDNA. The specific primers PMMoV-F PMMoV-R. The obtained cDNA was used as a template for PCR amplification, and the pepper light mottle mosaic virus shouguang isolate was studied. The results showed that: a molecular weight of about 576bp bands were obtained, and the gene sequencing was carried out after purification. By comparing the sequencing results, the homology of PMMoV isolates with many isolates was 99% ~ 100% .