目的探讨主穹窿蛋白(major vault protein,MVP)在颞叶癫大鼠模型脑组织的表达及其与难治性癫耐药是否相关。方法用氯化锂-匹鲁卡品制作颞叶癫模型,并将它分为药物有效组和耐药组;用免疫组化和Western blot法检测MVP表达。结果MVP主要表达在海马和皮质区的小胶质细胞,血管内皮细胞,血管周围的星形胶质细胞也有表达,神经元中少见表达;耐药组MVP表达较药物有效组和对照组明显增高,具有统计学意义(P<0.05)。结论耐药大鼠模型中MVP过量表达,提示MVP可能与难治性癫的耐药性有关。 Objective To investigate the expression of major vault protein (MVP) in temporal lobe epilepsy rat model and its relationship with refractory epilepsy. Methods Temporal lobe epilepsy model was made with lithium chloride-pilocarpine and divided into two groups: drug-effective group and drug-resistant group. MVP expression was detected by immunohistochemistry and Western blot. Results MVP was mainly expressed in microglia, vascular endothelial cells and vascular astrocytes in the hippocampus and cortex regions, but also in neurons. MVP expression in drug-resistant group was significantly higher than that in drug-effective group and control group , With statistical significance (P <0.05). Conclusions The overexpression of MVP in the drug-resistant rat model suggests that MVP may be related to drug resistance in refractory epilepsy.