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目的探讨灯盏花素对抗结核药致小鼠肝损伤的保护作用及其机制。方法 40只小鼠随机分为对照组、模型组、护肝片组、灯盏花素高和低剂量共5组。除对照组外,其余各组每天采用异烟肼和利福平灌胃造成肝损伤模型,2 h后,对照组和模型组分别予以等体积生理盐水灌胃,其余各组分别予以护肝片、灯盏花素灌胃,连续2周。分别检测各组小鼠肝指数和血清谷丙转氨酶(ALT)的活性;肝组织匀浆丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性及肝组织病理学变化。结果灯盏花素和护肝片组均可明显降低肝损伤小鼠肝指数、血清ALT活性、肝匀浆MDA的含量,并能显著提高肝匀浆SOD的活性。肝组织病理学检查,灯盏花素组明显减轻肝细胞变性和坏死。结论灯盏花素对抗结核药致小鼠肝损伤具有明显的保护作用,其机制可能与其抗氧化作用有关。
Objective To investigate the protective effect and its mechanism of breviscapine against liver damage induced by tuberculosis in mice. Methods Forty mice were randomly divided into control group, model group, Hugan tablet group, breviscapine high and low dose group. In addition to the control group, the other groups every day with isoniazid and rifampin gavage caused liver injury model, 2 h after the control group and model group were given equal volume of normal saline gavage, and the remaining groups were given liver tablets , Breviscapine gavage for 2 weeks. The liver index and ALT activity were measured in each group. The contents of malondialdehyde (MDA), superoxide dismutase (SOD) and liver histopathological changes in liver homogenate were detected. Results Breviscapine and Hugan tablet group could significantly reduce the hepatic index, serum ALT activity, MDA content in liver homogenate, and significantly increase the SOD activity in liver homogenate. Liver histopathology, breviscapine group significantly reduce liver cell degeneration and necrosis. Conclusion Breviscapine has a significant protective effect on liver injury induced by TB in mice, and its mechanism may be related to its antioxidative effect.