论文部分内容阅读
通过改进的Hummers法制备氧化石墨烯(GO),利用酰胺化反应将端基为氨基的六臂聚乙二醇(PEG)连到氧化石墨烯表面,改善其水溶性和生物相容性.原子力显微镜(AFM)数据表明所制备的GO-PEG尺寸小于250 nm,稳定性试验证明GO-PEG在水和PBS缓冲液中可以很好地分散.利用制备的GO-PEG作为药物载体,通过物理共混的方法负载疏水性抗肿瘤药物——冬凌草甲素.紫外光谱法测得载药率高达105%,远高于一般其他的药物载体.选择肺癌细胞A549和乳腺癌细胞MCF-7对载药体系的细胞毒性进行了研究,结果表明即使在高达100 mg/L的浓度下培养48 h,载体GO-PEG对两种细胞仍然具有很小的毒性(相对细胞存活率>85%),而通过载体负载后冬凌草甲素的疗效有所增强,对细胞具有更大的杀伤作用.
Graphene oxide (GO) was prepared by the modified Hummers method, and six-arm polyethylene glycol (PEG) with amino group at the end was connected to the surface of graphene oxide by amidation to improve its water solubility and biocompatibility. Microscopy (AFM) data showed that the prepared GO-PEG was less than 250 nm in size and the stability test demonstrated that GO-PEG could be well dispersed in water and PBS buffer.With the prepared GO-PEG as drug carrier, Mixed method of hydrophobic drug loading hydrophobic load - Rubescensine A. UV spectroscopy measured drug loading rate as high as 105%, much higher than the average of other drug carriers.Selecting lung cancer cells A549 and breast cancer cells MCF-7 The cytotoxicity of the drug-loaded system was studied. The results showed that the GO-PEG carrier has little toxicity (relative cell viability> 85%) to both cells even after 48 h culture at a concentration up to 100 mg / L, After the load through the support of oridonin efficacy has been enhanced, the cells have a greater killing effect.