论文部分内容阅读
鹅掌楸属树种是珍稀的第三纪孑遗树种,也是优良用材兼园林绿化树种。但其分子生物学研究进展缓慢,全基因组的破译成为鹅掌楸属树种分子生物学研究深入开展的关键。本研究基于Illumina测序平台首次对鹅掌楸基因组的大小进行测定,通过生物信息学方法对其重复序列情况和杂合率等基本信息进行了预估,并进行了初步组装。主要结果如下:(1)鹅掌楸全基因组大小预估为1.57 Gb;(2)鹅掌楸基因组的杂合率和重复序列比例分别为0.93%和58.58%;(3)鹅掌楸属于高重复高杂合基因组,建议后续可以采用二代+三代(Illumina+Pac Bio)测序技术相结合的策略,辅以Hi-C技术以及相应的拼接软件,有利于鹅掌楸高质量全基因组图谱的获得。
Liriodendron species is a rare Tertiary relict species, but also an excellent timber and landscape tree species. However, its progress in molecular biology has been slow, and deciphering the whole genome has become the key to the in-depth study of molecular biology in the genus Liriodendron. In this study, the size of the Liriodendron malabaricus genome was determined for the first time based on the Illumina sequencing platform. Basic information such as its repeat sequence and heterozygosity was predicted by bioinformatics methods and preliminarily assembled. The main results are as follows: (1) The whole genome size of Liriodendron chinense is estimated to be 1.57 Gb. (2) The ratios of heterozygosity and repeats of Liriodendron chinense genome are 0.93% and 58.58%, respectively. (3) Repeatedly high heterozygous genomes suggested that follow-up can be combined with Illumina + Pac Bio sequencing technology, combined with Hi-C technology and the corresponding splicing software, is conducive to high quality whole genome map of Liriodendron obtain.