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目的:研究福莫特罗、吲哚美辛和罗红霉素对癌症恶病质小鼠的联合治疗作用,并探讨其可能机制。方法:将小鼠结肠腺癌Colon26(C26)细胞接种于雄性Babl/c小鼠皮下后9d,建立癌症恶病质模型。随机分为九组:A组(正常对照组)、B组(生理盐水治疗组)、C组(福莫特罗治疗组)、D组(吲哚美辛治疗组)、E组(罗红霉素治疗组)、F组(福莫特罗+吲哚美辛治疗组)、G组(福莫特罗+罗红霉素治疗组)、H组(吲哚美辛+罗红霉素治疗组)、I组(福莫特罗+吲哚美辛+罗红霉素治疗组)。观察癌症恶病质小鼠治疗后的体质量、去瘤体质量、食物摄入量、左侧腓肠肌重量和血清清蛋白、血糖、三酰甘油、TNF-α、IL-6水平。结果:荷瘤小鼠去瘤体质量明显下降,清蛋白和血糖水平明显降低,三酰甘油、TNF-α、IL-6水平升高。三药联合治疗组与其他组相比,左侧腓肠肌重量增加,血糖升高,三酰甘油降低,血清TNF-α降低。结论:福莫特罗、吲哚美辛和罗红霉素在一定程度上均具有抗癌症恶病质的作用,三药联合治疗恶病质效果更好。
Objective: To study the combined effect of formoterol, indomethacin and roxithromycin on cancer cachexia in mice and its possible mechanism. Methods: Mouse colon adenocarcinoma Colon26 (C26) cells were subcutaneously inoculated into male Babl / c mice for 9 days to establish models of cancer cachexia. Were randomly divided into nine groups: group A (normal control group), group B (saline treatment group), group C (formoterol treatment group), group D (indometacin treatment group), group E F group (formoterol + indometacin treatment group), G group (formoterol + roxithromycin treatment group), H group (indomethacin + roxithromycin group) Treatment group), group I (formoterol + indomethacin + roxithromycin treatment group). The body mass, tumor removal mass, food intake, left gastrocnemius muscle weight, serum albumin, blood glucose, triglyceride, TNF-α and IL-6 levels were observed after treating cancer cachexia mice. Results: The tumor mass of tumor-bearing mice was significantly decreased, albumin and blood glucose levels were significantly decreased, triglyceride, TNF-α, IL-6 levels were increased. Compared with the other three groups, the weight of the left gastrocnemius muscle increased, the blood sugar increased, the triglyceride decreased and the serum TNF-α decreased. CONCLUSION: Formoterol, indomethacin and roxithromycin all have anti-cancer cachexia effect to a certain extent. The combination of three drugs has better effect on cachexia.