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在氨水溶液中进行Fe~(+2)和Fe~(+3)离子共沉淀并水热处理后制得磁性纳米颗粒Fe_3O_4,通过戊二醛活化将纤维素酶固定于其上.采用基于响应面法的Box-Behnken法(BBD)优化了制备条件,如磁性纳米颗粒浓度、戊二醛浓度、酶浓度和交联时间.BBD分析结果表明,用实验数据可合理调节二次模型.利用生成的基于统计数据的等高线评价了响应面的变化,以理解纳米颗粒和酶活性之间的关系.运用扫描电镜、X射线衍射和红外光谱表征了纳米颗粒上酶的尺寸、结构、形貌和结合情况.采用诸如pH值、温度、重复使用性和存储能力分析了固定化纤维素酶的活性和稳定性.发现固定后的纤维素酶表现出更好的稳定性和活性.
The Fe ~ (+2) and Fe ~ (+3) ions were coprecipitated and hydrothermally treated in aqueous ammonia to prepare magnetic nanoparticles Fe_3O_4, and the cellulase was immobilized on it by glutaraldehyde activation.According to the response surface The method of Box-Behnken (BBD) optimizes the preparation conditions such as magnetic nanoparticle concentration, glutaraldehyde concentration, enzyme concentration and crosslinking time.BBD analysis results show that the experimental data can be used to adjust the quadratic model reasonably. The change of response surface was evaluated based on the contour of statistical data to understand the relationship between nanoparticles and enzyme activity.The size, structure, morphology and enzyme characterization of the nanoparticles on the nanoparticles were characterized by scanning electron microscopy, X-ray diffraction and infrared spectroscopy In combination, the activity and the stability of the immobilized cellulase were analyzed using factors such as pH, temperature, reusability and storage ability, and the immobilized cellulase was found to show better stability and activity.