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目的:观察用流式细胞仪(FCM)测定玉郎伞多糖(YLSP)对Aβ25-35诱导PC12细胞凋亡的影响。方法:采用Aβ25-35诱导PC12细胞凋亡损伤模型,将石杉碱甲作为对照药,进行YLSP预处理后用FCM测定各组细胞的凋亡率。结果:Aβ25-35作用于PC12细胞后,PC12细胞的24h或48h凋亡率均显著升高(P<0.01),经YLSP处理后,PC12细胞的24h或48h凋亡率显著降低(P<0.05或P<0.01),其中,YLSP高剂量组PC12细胞的24h或48h凋亡率明显低于石杉碱甲组(P<0.01)。结论:YLSP对Aβ25-35诱导PC12细胞的凋亡具有较好的保护作用。
AIM: To observe the effect of YLSP on the apoptosis of PC12 cells induced by Aβ25-35 by flow cytometry (FCM). Methods: Aβ25-35 induced PC12 cell apoptosis model, Huperzine A as a control drug, YLSP pretreatment with FCM to determine the apoptosis rate of each group of cells. Results: After treated with Aβ25-35, the apoptosis rate of PC12 cells was significantly increased at 24h or 48h (P <0.01). After treated with YLSP, the apoptosis rates of PC12 cells at 24h or 48h were significantly decreased (P <0.05 Or P <0.01). The apoptosis rate of PC12 cells in YLSP high dose group at 24h or 48h was significantly lower than that of huperzine A group (P <0.01). Conclusion: YLSP has good protective effect on Aβ25-35 induced PC12 cell apoptosis.