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目的 探讨大剂量免疫球蛋白治疗癫痫的机制及其与海马区白细胞介素 2受体的关系。方法 用腹腔注射美解眠制作大鼠癫痫模型 ,致痫前 2小时分别给予生理盐水和不同剂量的免疫球蛋白G ,隔日1次 ,共 4次。观察大鼠出现癫痫发作的潜伏期及惊厥评分 ,并用免疫组织化学方法观察免疫球蛋白G对癫痫大鼠海马区IL 2Rβ免疫反应阳性细胞的影响。 结果 致痫前给予免疫球蛋白G组大鼠癫痫发作潜伏期较对照组大鼠延长 (P <0 .0 0 1) ,惊厥评分降低 (P <0 .0 1) ,海马区IL 2Rβ免疫反应阳性细胞数减少 (P <0 .0 0 1)。结论 大剂量免疫球蛋白对癫痫发作具有肯定的抑制作用 ,其机制与该疗法调节中枢神经系统免疫反应及海马区IL 2R表达 ,并通过IL 2维持免疫 神经 内分泌网络的平衡有关。
Objective To investigate the mechanism of high-dose immunoglobulin in the treatment of epilepsy and its relationship with interleukin-2 receptor in hippocampus. Methods The rat model of epilepsy was made by intraperitoneal injection of US Jiemian. The rats were given normal saline and different doses of immunoglobulin G two hours before epileptic seizure, one time every other day for 4 times. The latency and seizure scores of seizures in rats were observed. Immunohistochemistry was used to observe the effect of immunoglobulin G on IL 2Rβ immunoreactive cells in hippocampus of epileptic rats. Results The latency of epileptic seizure induced by immunoglobulin G group was longer than that of the control group (P <0.01), the seizure score decreased (P <0.01), and the immunoreactivity of IL 2Rβ in hippocampus The number of cells decreased (P <0.01). Conclusions High-dose immunoglobulin has a positive inhibitory effect on epileptic seizures and its mechanism is related to the regulation of central nervous system immune response and IL-2R expression in hippocampus by the therapy and the balance of immune neuroendocrine network through IL 2.