Prokaryotic Expression and Polyclonal Antibody Preparation of PRL3

来源 :Chemical Research in Chinese Universities | 被引量 : 0次 | 上传用户:armstronger7026
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Phosphatase of regenerating liver 3(PRL3),which belongs to the superfamily of protein tyrosine phosphatases(PTPs),represents a group of low molecular weight PTPs that participate in tumorigenesis and metastasis processes.Presented here are the results of cloning,prokaryotic expression,purification,and polyclonal antibody preparation of PRL3.To obtain a specific polyclonal antibody against PRL3,the authors have prepared GST-PRL3 to immunize rabbits and purify an anti-PRL3 polyclonal antibody by negative selection affinity columns.Western blot analysis shows that the anti-PRL3 polyclonal antibody has a specific binding ability with PRL3 protein.The anti-PRL3 polyclonal antibody provides a good tool to further study the function of PRL3. Phosphatase of regenerating liver 3 (PRL3), which belongs to the superfamily of protein tyrosine phosphatases (PTPs), represents a group of low molecular weight PTPs that participate in tumorigenesis and metastasis processes. Presented here are the results of cloning, prokaryotic expression, purification , and polyclonal antibody preparation of PRL3.To obtain a specific polyclonal antibody against PRL3, the authors have prepared GST-PRL3 to immunize rabbits and purify an anti-PRL3 polyclonal antibody by negative selection affinity columns. Western blot analysis shows that the anti-PRL3 polyclonal antibody has a specific binding ability with PRL3 protein. The anti-PRL3 polyclonal antibody provides a good tool to further study the function of PRL3.
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