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目的 建立检测人血浆中劳拉西泮浓度的高效液相色谱方法。方法 血浆经乙醚萃取浓缩 ,以ZORBAXEclipseXDB C8(15 0mm× 4 .6mm ,5 μm)为色谱柱 ;以乙腈 水 (38.8∶6 1.2 )为流动相 ,流速为 1.0mL·min-1;以紫外 2 30nm为检测波长。结果 劳拉西泮浓度在 5 .0 0～ 15 0 .0 0 μg·L-1内线性关系良好 (r =0 .9993) ;最低检测限为 2 .5 0 μg·L-1;平均回收率为 (99.0 8±6 .86 ) % ,日内、日间RSD分别为 (5 .4 0± 3.32 ) %和 (7.35± 3.13) %。内标物地西泮的绝对回收率为 (78.87± 4 .2 9) % ;峰面积变异为 5 .4 8%。结论 方法简便、快速、准确可靠 ,适用于劳拉西泮血浓度的测定和药代动力学研究。
Objective To establish a HPLC method for the determination of lorazepam in human plasma. Methods The plasma was concentrated by diethyl ether and chromatographed on ZORBAXEclipseXDB C8 (15 0 mm × 4 .6 mm, 5 μm) column. The mobile phase consisted of acetonitrile and water (38.8: 6.2) with a flow rate of 1.0 mL · min- 30nm for the detection wavelength. Results The linearity of lorazepam was in the range of 5.0 ~ 150.0 μg · L-1 (r = 0.9993) and the lowest detection limit was 2.50 μg · L-1. The average recovery The rate was (99.0 8 ± 6. 86)%, and the intraday and interday RSD were (5.40 ± 3.32)% and (7.35 ± 3.13)%, respectively. The internal standard diazepam absolute recovery rate was (78.87 ± 4.29)%; peak area variation was 5.58%. Conclusion The method is simple, rapid, accurate and reliable, suitable for the determination of lorazepam blood concentration and pharmacokinetic studies.