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目的 :获得pS2融合蛋白。方法 :RT PCR法从乳腺癌组织中扩增pS2片段 ,定向克隆到PMS 31b载体上 ,重组质粒PMS pS2转化温度敏感型细菌POP2 136 ,使之高效表达融合蛋白MS2 pS2
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